violetFluor™ 450 Anti-Human CD45RA (HI100) Antibody
Catalogue Number: ATB10358-ABG
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | Receptor-type tyrosine-protein phosphatase C, 3.1.3.48, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | HI100 |
| Isotype: | IgG2b, kappa |
| Immunogen: | |
| Application: | FC |
Description
Description: The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB and CD45RO.The HI100 antibody is widely used as a marker for human CD45RA expression on nave and activated T cells, B cells, and monocytes.
Additional Text
Gene Name
PTPRC
Gene ID
5788
Uniprot ID
P08575
Application Notes
This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.
Antibody Clonality
Monoclonal
Storage Note
2-8°C protected from light
ATB10358 Datasheet
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