BACH1 (APC)
Catalogue Number: B0003-04-APC-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Allophycocyanin (APC). |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 3G11 |
| Isotype: | IgG1 |
| Immunogen: | BACH1/GST fusion protein encompassing the C-terminus of human BACH1. |
| Application: | IF, IP, WB |
Description
Description: BRCA1 has been shown to interact with a novel member of the DEAH helicase family, BACH12. The BRCA1 gene encodes an 1863 residue nuclear protein with N-terminal RING and C-terminal BRCT domains. BACH1 appears to bind directly to these BRCT repeats of BRCA1. Moreover, BACH1 appears to contribute to the DNA repair of BRCA1. BACH1 derivative bearing a mutation in a key residue that is essential for catalytic function in other helicases interfered with normal DSBR in a manner that was dependent upon its ability to interact with BRCA12. Tumorpredisposing missense and deletion mutations in the BRCA1 BRCT domain, all of which render BRCA1 defective in its DSBR function also disrupted BACH1 binding to BRCA1. BACH1 is an integral part of BRCA1 in DSBR2. Western blots of extracts from three different cell lines revealed a band of 130kD for BACH1 in all MCF7, HeLa and HCC1937 cell line. Immunofluorescence analysis revealed the presence of punctate nuclear staining in multiple human cell lines.
Additional Text
Specificity
Recognizes the ~130kD human BACH1 protein.
Purification
Protein A purified
Antibody Clonality
Monoclonal
B0003-04-APC Datasheet
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