Anti-Human BCL-2 (124) - Purified No Carrier Protein
Catalogue Number: B130-LET
| Manufacturer: | Leinco Technologies, Inc |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg, 100 ug, 25 ug, 500 ug |
| Host name: | |
| Clone: | 124 |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA, FC, IF, IHC-P, IP, WB, IHC |
Description
Description: BCL-2 is a member of a protein family that regulates apoptosis, with some members inducing (proapoptotic) and others inhibiting (antiapoptotic) apoptosis 1. BCL-2 acts as an antiapoptotic protein that prevents apoptosis and promotes survival in response to many cell stresses and cytotoxic chemicals 2 via the mitochondria-mediated intrinsic apoptosis pathway 1. BCL-2 is also an important oncogene whose overexpression is common in hematological malignancies such as follicular lymphoma, chronic lymphocytic leukemia, mantle cell leukemia and Waldenstroem's macroglobulinemia 1 as well as breast cancer 3. As such, BCL-2 is a target for inhibition in cancer immunotherapy. Additionally, BCL-2 is involved in cardiac hypertrophy and has been suggested as a potential therapeutic target for clinical management 4. Clone 124 was generated by immunizing mice with a synthetic peptide corresponding to amino acids 41 to 54 (GAAPAPGIFSSQPG) of BCL-2 conjugated to thyroglobulin 5. Sera were screened on cryostat sections of human tonsil and on a follicular lymphoma with a known 14;18 translocation. Cell fusion and hybridoma lines were produced by conventional technology. 124 recognizes BCL-2 by Western blotting under both reducing and non-reducing conditions.
Additional Text
Purification
Purified
Antibody Clonality
Monoclonal
Concentration
1 mg/ml
Storage Note
This purified antibody is stable when stored at 2-8°C. Do not freeze.
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations