Mouse anti Human CD4 FITC - HLADR PE

Catalogue Number: B4DRS-EXA

Manufacturer:ExAlpha
Type:Primary Antibody
Alias:CD4 FITC - HLADR PE
Shipping Condition:Blue Ice
Unit(s): 50 tests
Host name: Mouse
Clone:
Isotype:
Immunogen: CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Application: FC

Description

Description: CD4 identifies human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. HLA-DR identifies human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.

Additional Text

Gene Name

CD4

Gene ID

920

Uniprot ID

P01730

Purification

Protein A/G Purified

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE