5-Methyl-CTP
Catalogue Number: B7967-APE
| Manufacturer: | Apexbio |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Alias: | 5-Methylcytidine-5'-Triphosphate, 5-Me-CTP, 5mCTP, 5-mCTP |
| Shipping Condition: | Dry Ice |
| Storage Condition: | -20°C/-70°C |
| Unit(s): | 10 ul (100 mM), 100 ul (100 mM), 50 ul (100 mM) |
Description
Description: 5-Methyl-CTP is a modified nucleoside triphosphate, commonly used to substitute the naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis. Methylation of the cytosine C-5 position is a common, post-transcriptional modification for mRNA. Incorporation of chemically modified 5-methyl-CTP can increase mRNA stability and protein expression, and meanwhile, reduce interaction of innate immune receptors with in vitro-transcribed (IVT) mRNA. Apart from its application in encoding antigens for the activation of immune response against a variety of diseases such as COVID-19, IVT mRNA can also express the nanobody/variable domain of heavy chain of heavy-chain antibody targeting the intracellular components, enabling intracellular antigen labeling and manipulation. References:1. Rahman MM, Zhou N, Huang J. An overview on the development of mRNA-based vaccines and their formulation strategies for improved antigen expression in vivo. Vaccines (Basel), 2021, 9(3): 244.2. Lu J, Lu G, Tan S, et al. A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice. Cell Research, 2020, 30(10): 936-939.3. Zhou X, Hao R, Chen C, et al. Rapid delivery of nanobodies/VHHs into living cells via expressing in vitro-transcribed mRNA. Molecular Therapy - Methods & Clinical Development, 2020, 17: 401-408.
Additional Text
Reagent:Smile String
[NH2]=C(C(C)=CN1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)=NC1=O.[Li+].[Li+].[Li+].[Li+]
Storage Note
Store at -20°C or below
Short Description
A CTP analogue used for in vitro mRNA synthesis
Molecular Formula
C10H18N3O14P3 (free acid)
Molecular Weight
497.1 (free acid)
B7967 CofA
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