5-Methoxy-UTP
Catalogue Number: B8061-APE
| Manufacturer: | Apexbio |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Alias: | 5-Methoxyuridine-5'-Triphosphate, 5-moUTP |
| Shipping Condition: | Dry Ice |
| Storage Condition: | -20°C/-70°C |
| Unit(s): | 10 ul, 100 ul, 10x100 ul, 50 ul, 5x100 ul |
Description
Description: 5-Methoxy-UTP is an analogue of the naturally occurring uridine triphosphate (UTP). Similar to N1-methylpseudouridine and pseudouridine modifications, 5-methoxyuridine is another common chemical modification used for in vitro mRNA synthesis in order to improve mRNA activity and reduce innate immune responses. Chemical modifications of mRNAs with 5-methoxyuridine, N1-methylpseudouridine and pseudouridine have been shown to significantly improve the expression of enhanced green fluorescent protein (eGFP) in a variety of cell lines, among which 5-methoxyuridine-modified eGFP mRNA is more stable than other eGFP mRNAs. In addition, replacing UTP with 5-methoxy-UTP in Cas9 mRNA has also been reported to reduce innate immune responses, making the broad use of Cas9 possible both in vitro and in vivo. References:1. Li B, Luo X, Dong Y. Effects of Chemically Modified Messenger RNA on Protein Expression. Bioconjugate Chemistry, 2016, 27(3): 849-853.2. Vaidyanathan S, Azizian KT, Haque AKMA, et al. Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification. Molecular Therapy - Nucleic Acids, 2018, 12: 530-542.
Additional Text
Reagent:Smile String
O=C(C(OC)=CN1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)NC1=O.[Li+].[Li+].[Li+].[Li+]
Storage Note
Store at -20°C or below
Short Description
A UTP analogue, commonly used for in vitro mRNA synthesis
Molecular Formula
C10H17N2O16P3 (free acid)
Molecular Weight
514.1 (free acid)
B8061 MSDS
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