Anti-PD-L1 [10F.9G2]
Catalogue Number: BAB0615-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD274; PDL1; PD L1; Programmed cell death 1 ligand 1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; 10F9G2; 10F 9G2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Mouse |
| Clone: | 10F.9G2 |
| Isotype: | IgG2a |
| Immunogen: | This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD-L1 CHO transfectants. |
| Application: | ELISA, FC, IHC, Blk |
Additional Text
Gene Name
CD274
Uniprot ID
Q9EP73
Gene ID
60533
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011).
Short Description
This is a bispecific, knob-into-hole heterodimerized antibody, with one arm directed against mouse PD-L1 (Fab) and one arm directed against mouse CD3e (scFv).
BAB0615-BT Datasheet
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