Nestin Polyclonal Antibody
Catalogue Number: BS-0008R-BSS
BS-0008R Image
BS-0008R Image
BS-0008R Image
BS-0008R Image
BS-0008R Image
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Nestin Polyclonal Antibody (bs-0008R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
BS-0008R Image
Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti-Nestin Polyclonal Antibody (bs-0008R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
BS-0008R Image
Tissue/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nestin) polyclonal Antibody, Unconjugated (bs-0008R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
BS-0008R Image
Neuro-2a cells probed with Rabbit Anti-Nestin Polyclonal Antibody (bs-0008R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat Nestin |
| Application: | ELISA, FC, ICC, IF, IHC-P, IHC-Fr |
Description
Description: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Additional Text
Gene Name
NES
Gene ID
25491
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Uniprot ID
P21263
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
BS-0008R Datasheet
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