Osteopontin Polyclonal Antibody
Catalogue Number: BS-0026R-BSS
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Formalin-fixed and paraffin-embedded rat hippocampus labeled with Rabbit Anti-OPN Polyclonal Antibody, Unconjugated (bs-0026R) at 1:300 followed by conjugation to the secondary antibody
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HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteopontin Polyclonal Antibody(bs-0026R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Osteopontin Polyclonal Antibody, Unconjugated (bs-0026R) at 1:400 overnight at 4°C, DAB staining.
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Formalin-fixed and paraffin embedded rat shin bone labeled with Anti-Osteopontin Polyclonal Antibody, Unconjugated (bs-0026R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
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Mouse kidney lysates probed with (bs-00265R) at 1:300 overnight in 4°C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1; Osteopontin; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; Secreted phosphoprotein 1; Spp1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse OPN |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-1 and is essential in the pathway that leads to type I immunity.
Additional Text
Gene Name
SPP1
Uniprot ID
P10923
Gene ID
20750
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0026R Datasheet
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