CASP8 Polyclonal Antibody
Catalogue Number: BS-0052R-BSS
bs-0052R Image
bs-0052R Image
bs-0052R Image
bs-0052R Image
bs-0052R Image
bs-0052R Image
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
bs-0052R Image
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
bs-0052R Image
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase 8) polyclonal Antibody, Unconjugated (bs-0052R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
bs-0052R Image
Image provided by the Independent Validation Program (badge number 29759). Lane 1: HeLa cell extract, Lane 2: c6/36 mosquito cell extract (non-reactive species) probed with Rabbit Anti-Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26°C.
bs-0052R Image
L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 12kD. Observed band size:48kD.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | MACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat CASP8 subunit p10 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Additional Text
Gene Name
CASP8
Purification
Protein A purified
Uniprot ID
Q9JHX4
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID
12370; 64044
bs-0052R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations