beta-Actin Polyclonal Antibody

Catalogue Number: BS-0061R-BSS

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A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4°C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution and 60 min incubation at 37°C.

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Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Lane 1: Mouse Lung lysates; Lane 2: Mouse Cerebrum lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Lane 1: Mouse Thymus lysates; Lane 2: Mouse Urinary bladder lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Aorta lysates ; Lane 5: Mouse olfactory bulb lysates; Lane 6: Mouse Cerebellum lysates; Lane 7: Mouse Adrenal gland lysates; Lane 8: Mouse Ovary lysates; Lane 9: Mouse Ear lysates; Lane 10: U2os cell lysates; Lane 11: ASPC-1 cell lysates ; Lane 12: Mouse Vas deferens lysates; Lane 13: Mouse trachea lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Lane 1: Mouse Embryo Cerebrum lysates; Lane 2: Human Du145 cell lysates; Lane 3: Human SW480 cell lysates; Lane 4: Human U87MG cell lysates ; Lane 5: Human U251 cell lysates; Lane 6: Human A673 cell lysates; Lane 7: Human Lovo cell lysates; Lane 8: Human 293FT cell lysates; Lane 9: Human JEG-3 cell lysates; Lane 10: Rat RSC96 cell lysates; Lane 11: Human MCF-7 cell lysates ; Lane 12: Human HepG2 cell lysates; Lane 13: Human A431 cell lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Lane 1: A549 cell lysates; Lane 2: Raw264.7 cell lysates; Lane 3: SH-SY5Y cell lysates; Lane 4: MKN45 cell lysates ; Lane 5: CHO cell lysates; Lane 6: Panc-1 cell lysates; Lane 7: 4T1 cell lysates; Lane 8: ASPC-1 cell lysates; Lane 9: H9C2 cell lysates; Lane 10: Brl-3a cell lysates; Lane 11: TT cell lysates ; Lane 12: TEV-1 cell lysates; Lane 13: EC9706 cell lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Lane 1: Human HL60 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Rat Spinal cord lysates; Lane 4: Rat Rectum lysates ; Lane 5: Rat Placenta lysates; Lane 6: Rat Lymph node lysates; Lane 7: Rat Lung lysates; Lane 8: Rat Spleen lysates; Lane 9: Human JAR cell lysates; Lane 10: Human 293T cell lysates; Lane 11: Human Jurkat cell lysates ; Lane 12: Human TM4 cell lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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NIH/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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Sample: Lymph node (Rat) Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/1000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD

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Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/1000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD

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Sample: SH-SY5Y (Human) Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/2000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD

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Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.

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Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4°C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.

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Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Paraformaldehyde-fixed, paraffin embedded Mouse Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BS-0061R Image

Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	BRWS1; PS1TP5BP1; Actin, cytoplasmic 1; Beta-actin; ACTB
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	MAP conjugated synthetic peptide derived from human beta Actin
Application:	ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr

Overview
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Description

Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.