CD19 Polyclonal Antibody
Catalogue Number: BS-0079R-BSS
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Paraformaldehyde-fixed, paraffin embedded mouse embryo tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD19 Polyclonal Antibody, Unconjugated (bs-0079R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Human Raji cells probed with CD19 Polyclonal Antibody, Unconjugated (bs-0079R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-CD19 Polyclonal Antibody (bs-0079R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
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Mouse spleen lysate probed with Rabbit Anti-CD19 Polyclonal Antibody, Unconjugated (bs-0079R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 59kD. Observed band size: 28kD and 90kD
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | AW495831; B-lymphocyte antigen CD19; Differentiation antigen CD19; CD19 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat CD19 |
| Application: | ELISA, FC, ICC, IF, IHC-P, IHC-Fr |
Description
Description: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Additional Text
Gene Name
CD19
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Uniprot ID
F1LNH2
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID
12478; 365367
bs-0079R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations