p75 NGF Receptor Polyclonal Antibody
Catalogue Number: BS-0161R-BSS
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Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C
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Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
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HepG2 cells were stained with p75 NGF receptor Polyclonal Antibody, Unconjugated(bs-0161R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
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Mouse spleen cells probed with p75 NGF Receptor Polyclonal Antibody, unconjugated (bs-0161R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
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Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NGF Receptor) polyclonal Antibody, Unconjugated (bs-0161R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei
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Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated (bs-0161R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Mouse brain lysates probed with Anti-p75 NGF Receptor Polyclonal Antibody (bs-0161R) at 1:300 overnight in 4°C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | p75; LNGFR; p75NTR; RNNGFRR; Tnfrsf16; Tumor necrosis factor receptor superfamily member 16; Gp8-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; CD271; Ngfr |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat NGFR |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake. Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.
Additional Text
Gene Name
NGFR
Uniprot ID
P07174
Gene ID
24596
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0161R Datasheet
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