Fas Ligand Polyclonal Antibody
Catalogue Number: BS-0216R-BSS
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Jurkat lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200 overnight at 4°C, DAB staining.
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Lane 1: Mouse Bone lysates; Lane 2: Rat Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1; Tumor necrosis factor ligand superfamily member 6; Apoptosis antigen ligand; CD95 ligand; Fas antigen ligand; Fas ligand; FASLG |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Fas Ligand |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.
Additional Text
Gene Name
FASLG
Uniprot ID
P48023
Gene ID
356
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0216R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations