IFN gamma Polyclonal Antibody
Catalogue Number: BS-0480R-BSS
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BS-0480R Image
BS-0480R Image
BS-0480R Image
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BS-0480R Image
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BS-0480R Image
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Lane 1: Mouse Raw264.7-PMA Lysates; Lane 2: Mouse Lymph Lysates; Lane 3: Mouse Spleen Lysates; Lane 4: Mouse Thymus Lysates; Lane 5: Rat Lymph Lysates; Lane 6: Rat Thymus Lysates; Lane 7: Human Jurkat-TPA cell Lysates; Lane 8: Human Jurkat cell Lysates; Lane 9: Human HepG2 cell Lysates; Lane 10: Recombinant human IFN gamma Protein (100ng) (bs-0388P). Probed with IFN gamma polyclonal Antibody, unconjugated (bs-0480R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IFN-gamma Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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ctll-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IFN gamma) polyclonal Antibody, Unconjugated (bs-0480R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
BS-0480R Image
Paraformaldehyde-fixed, paraffin embedded (rat lymph); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
BS-0480R Image
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
BS-0480R Image
Paraformaldehyde-fixed, paraffin embedded (mouse lymph); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IFN gamma Antibody(bs-0480R)at 1:200 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Diseased mouse lung and spleen lysates probed with Anti-IFN gamma Polyclonal Antibody (bs-0480R) at 1:300 overnight in 4°C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
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25 ug total protein per lane of various lysates (see on figure) probed with IFN gamma polyclonal antibody, unconjugated (bs-0480R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Ifg; IFN-g; Interferon gamma; IFN-gamma; Ifng |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse IFN gamma |
| Application: | FC, ICC, IF, WB |
Description
Description: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Additional Text
Gene Name
IFNG
Uniprot ID
P01580
Gene ID
15978
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
BS-0480R Datasheet
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