Chromogranin A Polyclonal Antibody
Catalogue Number: BS-0539R-BSS
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bs-0539R Image
bs-0539R Image
BS-0539R Image
bs-0539R Image
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CGA/ Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 overnight at 4°C, DAB staining.
bs-0539R Image
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 overnight at 4°C, DAB staining.
BS-0539R Image
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Chromogranin A) polyclonal Antibody, Unconjugated (bs-0539R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CGA; Chromogranin-A; Pituitary secretory protein I; SP-I; CHGA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human CGA |
| Application: | ELISA, ICC, IF, IHC-P, IHC-Fr |
Description
Description: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008].
Additional Text
Gene ID
1113
Gene Name
CHGA
Uniprot ID
P10645
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0539R Datasheet
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