CCNB1 Polyclonal Antibody
Catalogue Number: BS-0572R-BSS
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HeLa lysates probed with Anti -(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37°C.
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U937 lysates probed with Anti -(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37°C.
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U251 lysates probed with Anti -(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37°C.
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K562 lysates probed with Anti -(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37°C.
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Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-Cyclin B1 Polyclonal Antibody (bs-0572R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Cyclin B1 Polyclonal Antibody (bs-0572R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Lane 1: Rat Lymph node lysates; Lane 2: Rat Testis lysates; Lane 3: Rat Spleen lysates probed with Cyclin B1 Polyclonal Antibody, Unconjugated (bs-0572R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin B1) polyclonal Antibody, Unconjugated (bs-0572R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Cyclin B1 Polyclonal Antibody, Unconjugated (bs-0572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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HeLa cells probed with Rabbit Anti-Cyclin B1 Polyclonal Antibody (bs-0572R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CycB1; Cycb-4; Cycb-5; Ccnb1-rs1; Cycb1-rs1; Ccnb1-rs13; G2/mitotic-specific cyclin-B1; Ccnb1; Ccn-2; Cycb |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human CCNB1 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Additional Text
Uniprot ID
P14635
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID
268697; 891
Gene Name
Ccnb1, CCNB1
bs-0572R Datasheet
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