CD4 Polyclonal Antibody
Catalogue Number: BS-0647R-BSS
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Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Lane 1: HL60 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Lane 1: Mouse Spleen; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Lane 1: Mouse Lymph Node; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Human HL60 Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 1 X PBS / 2% BSA / 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with CD4 Polyclonal Antibody Unconjugated (bs-0647R; green) at 1:100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-FITC (bs-0295G-FITC), was used for 40 min at room temperature. Primary antibody staining is compared to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).
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Lane 1: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Human U937 cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.
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Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.
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Image provided by the Independent Validation Program, badge number 029634:Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
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Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with (bs-0647R) at 1:300 overnight in 4°C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37°C.
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Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | T-cell surface glycoprotein CD4; Cd4; T-cell surface antigen T4/Leu-3; W3/25 antigen |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain) |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
Additional Text
Uniprot ID
P01730
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID
24932; 920
Gene Name
Cd4, CD4
bs-0647R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations