CD8 Polyclonal Antibody
Catalogue Number: BS-0648R-BSS
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
BS-0648R Image
Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
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Lane 1: Mouse Spleen; 40ug loaded in each lane; Probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.
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Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated(bs-0648R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Lane 1: Jurkat cell lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: mCD8a overexpression 293T cell lysates probed with CD8a Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:250.
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Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Lymph node tissue lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
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Molt-4 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD8 Antibody(bs-0648R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody (bs-0648R) at 1:300 overnight in 4°C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
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Paraformaldehyde-fixed, paraffin embedded mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-CD8 Antibody, conjugated (bs-0648R-AF555) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.
BS-0648R Image
Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: CD8 Antibody, conjugated (bs-0648R-AF488) 1:400, 90 minutes at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Lyt2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse CD8 |
| Application: | FC |
Description
Description: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Additional Text
Gene Name
CD8A
Gene ID
12525
Uniprot ID
P01731
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
BS-0648R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations