Fibronectin Polyclonal Antibody
Catalogue Number: BS-0666R-BSS
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Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Paraformaldehyde-fixed, paraffin embedded rat intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Formalin-fixed and rat pancreas tissue labeled with Anti-FN/Fibronectin 1 Polyclonal Antibody, Unconjugated (bs-0666R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)
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Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:200 overnight at 4°C, DAB staining.
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Image kindly submitted by Edita Aksamitiene as part of the free sample program. Rat skin lysates probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:1000.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Fn; Fn-1; E3327I9; Fibronectin; Fn1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse Fibronectin |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling (By similarity).
Additional Text
Gene Name
FN1
Gene ID
14268
UniProt ID
P11276
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0666R Datasheet
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DELIVERY
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