CD4 Polyclonal Antibody

Catalogue Number: BS-0766R-BSS

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Formalin-fixed and paraffin embedded rat skin labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) 1:300 followed by conjugation to the secondary antibody and DAB staining.

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Formalin-fixed and paraffin embedded mouse kidney cells labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0766R) 1:200 followed by conjugation to the secondary antibody and DAB staining.

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Formalin-fixed and paraffin embedded Lymphocytes labeled with (bs-0766R) Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated followed by incubation with a conjugated secondary antibody.

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Formalin-fixed and paraffin embedded Lymphocytes labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) followed by incubation with a conjugated secondary antibody and DAPI staining.

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Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0766R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

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Mouse spleen lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0766R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.

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Lane 1: Mouse Lymph Node; Lane 2: Mouse Spleen; 40ug loaded into each lane; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0766R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.

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Tissue/cell: Mouse Lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: CD4 Polyclonal Antibody, Unconjugated (bs-0766R) 1:200, overnight at 4°C, followed by incubation with secondary antibody and DAB staining

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Mouse Spleen Cells were fixed with 70% methanol (Overnight at 4°C). The cells were then incubated in 1X PBS / 2% BSA / 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Primary incubation with CD4 Polyclonal Antibody, PE-Conjugated (bs-0766R-PE) at 1:100 for 30 minutes at room temp. The graph compares the primary antibody (green) to unstained cells (dark blue) and isotype control (orange).

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Mouse spleen cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD4 Polyclonal Antibody(bs-0766R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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Hamster lung IF staining

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Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Raji cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0766R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	L3T4; Ly-4; T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; CD4
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	KLH conjugated synthetic peptide derived from mouse CD4
Application:	ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr