IL-1 Beta Polyclonal Antibody
Catalogue Number: BS-0812R-BSS
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Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C. Followed by incubation with the secondary antibody, DAB and counterstaining.
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Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C. Followed by incubation with a secondary antibody, DAB and counterstaining.
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Paraformaldehyde-fixed, paraffin-embedded Rat Lung tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C. Followed by incubation with a secondary antibody, DAB and counterstaining.
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Lane 1: Normal Mouse Raw264.7 cell lysates; Lane 2: Raw264.7 treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.
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Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | IL-1; IL1F2; IL1-BETA; Interleukin-1 beta; IL-1 beta; Catabolin; IL1B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human IL-1beta |
| Application: | WB |
Description
Description: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Additional Text
Gene Name
IL1B
Gene ID
3553
Uniprot ID
P01584
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
BS-0812R Datasheet
SUPPORT
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DELIVERY
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