Recombinant GST protein, Tag free
Catalogue Number: BS-0883P-BSS
| Manufacturer: | Bioss Inc |
| Type: | Transferases |
| Host Cell: | E.coli |
| Shipping Condition: | Blue Ice |
| Storage Condition: | -70°C |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA |
Description
Description: Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 22amino acids(roughly 26 KDa), which, compared to other tags like the Myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include athrombindomain for cleavage of the GST tag during protein purification.
Additional Text
Protein Tag
Untagged
Storage Note
The product should be stored at -70°C or -20°C.
Short Description
Recombinant GST protein, Tag free protein is expressed in E.coli with Tag free. It contains the amino acid sequence of 1-211/211.
Endotoxin
Not Tested
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