ADRB2 Polyclonal Antibody
Catalogue Number: BS-0947R-BSS
BS-0947R Image
BS-0947R Image
BS-0947R Image
BS-0947R Image
BS-0947R Image
BS-0947R Image
BS-0947R Image
BS-0947R Image
BS-0947R Image
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Rat Stomach tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Human A431 cell lysates probed with ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Mouse splenoctyes probed with Rabbit Anti-ADRB2 Polyclonal Antibody (bs-0947R) at 1:100 for 30 minutes followed by incubation with Anti-Rabbit IgG FITC conjugated secondary compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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L1 rat brain lysates L2 rat heart lysates probed with Anti ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 46kD. Observed band size: 46kD
BS-0947R Image
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,pemeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADRB2 Antibody (bs-0947R-A488) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
BS-0947R Image
Mouse kidney cells (black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADRB2 Polyclonal Antibody(bs-0947R-AF488)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BS-0947R Image
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADRB2 Polyclonal Antibody(bs-0947R-AF488)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BS-0947R Image
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADRB2 Polyclonal Antibody.ALEXA FLUOR® 647 Conjugated(bs-0947R-AF647)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | BAR; B2AR; ADRBR; ADRB2R; BETA2AR; Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor; ADRB2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human of human ADRB2 |
| Application: | WB |
Description
Description: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 3-fold greater affinity than it does norepinephrine.
Additional Text
Gene ID
154
Gene Name
ADRB2
Uniprot ID
P07550
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
BS-0947R Datasheet
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