AKT1 (Ser473 + Tyr474) Antibody, ALEXA FLUOR® 594 Conjugated

Catalogue Number: BS-10134R-A594-BSS

Manufacturer:Bioss Inc
Shelf Life:12 months
Preservative:0.09% sodium azide
Type:Polyclonal Primary Antibody - Conjugated
Shipping Condition:Blue Ice
Storage Condition:4°C
Unit(s): 100 ul
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473/Tyr474

Description

Description: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

Additional Text

Concentration

1ug/ul

Applications with dilutions

IF(IHC-P)(1:50-200)

Excitation/ Emission Wavelengths

590nm/617nm

Protein modification

Phosphorylation Ser473 + Tyr474

Modification

Phosphorylation

Modification Site

Ser473 + Tyr474

Synonym

AKT1phospho Ser473/Tyr474; AKT1 Ser473/Tyr474; AKT1 phospho S473; AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK Alpha; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1.