Collagen 1 Polyclonal Antibody
Catalogue Number: BS-10423R-BSS
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This image was kindly submitted by Kandy Velazquez of USC School of Medicine. Mouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.
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Lane 1: Mouse Ovary; Lane 2: NIH/3T3; Lane 3: Rat Liver; Lane 4: 293T; Lane 5: A431; Lane 6: RAW264.7; Probed with Collagen I Polyclonal Antibody (bs-10423R) at 1:300 dilution overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin-embedded Human Lung Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.
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Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.
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Lane 1: HepG2 cell lysates probed with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Mouse Placenta lysates; Lane 2: Human U-2OS cell lysates probed with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C
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Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Collagen I antibody (bs-10423R) Dilution: 1ug/Test; Protocol Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Collagen I Polyclonal Antibody(bs-10423R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. MCF-7 cells probed with Rabbit Anti-Collagen I Polyclonal antibody (bs-10423R)at 1:1000.
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Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. Rat aorta, brain, spleen, pancreas, muscle, bladder, lungs, liver, and kidney lysates probed with Rabbit Anti-Collagen IﾠPolyclonal antibody (bs-10423R)at 1:1000.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1l; CO1A1_HUMAN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Collagen I |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Type I collagen is a member of group I collagen (fibrillar forming collagen).
Additional Text
Gene Name
COL1A1
Uniprot ID
P02452
Gene ID
1277
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-10423R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations