Calpain 1 Polyclonal Antibody
Catalogue Number: BS-1099R-BSS
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Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated (bs-1099R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Paraformaldehyde-fixed, paraffin embedded mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated (bs-1099R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated (bs-1099R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
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Human MCF7 cells probed with Calpain 1 Polyclonal Antibody, unconjugated (bs-1099R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
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Mouse kidney and liver lysates probed with Calpain 1 Polyclonal Antibody, unconjugated (bs-1099R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calpain 1 ) Polyclonal Antibody, Unconjugated (bs-1099R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Capa1; Capa-1; mu-calpin; Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Micromolar-calpain; muCANP; Capn1; Canp1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse Calpain 1 |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, WB, IHC-Fr |
Description
Description: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Additional Text
Gene Name
CAPN1
Purification
Protein A purified
Uniprot ID
O35350
Gene ID
12333
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1099R Datasheet
SUPPORT
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DELIVERY
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