SHANK3 Polyclonal Antibody
Catalogue Number: BS-12143R-BSS
BS-12143R Image
BS-12143R Image
BS-12143R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SHANK3 Polyclonal Antibody, Unconjugated (bs-12143R) at 1:200 overnight at 4°C, DAB staining.
BS-12143R Image
Formalin-fixed and paraffin embedded rat brain labeled with Anti-SHANK3 Polyclonal Antibody, Unconjugated (bs-12143R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3; SH3 and multiple ankyrin repeat domains protein 3; Shank3; Proline-rich synapse-associated protein 2; KIAA1650 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human SHANK3 |
| Application: | ELISA, ICC, IF, IHC-P, IHC-Fr |
Description
Description: Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.
Additional Text
Uniprot ID
Q9BYB0
Gene Name
SHANK3
Gene ID
85358
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-12143R Datasheet
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