GRP78 Polyclonal Antibody
Catalogue Number: BS-1219R-BSS
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Image provided by One World Lab validation program. SMMC7721 cells probed with Rabbit Anti-GRP78 Polyclonal Antibody (bs-1219R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Image provided by One World Lab validation program. PC3 cells probed with Rabbit Anti-GRP78 Polyclonal Antibody (bs-1219R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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U87MG cell lysates probed with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Rat Liver lysates probed with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Rat Spleen lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human MDA-MB-231 cell lysates; Lane 7: Human A431 cell lysates probed with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:400 overnight at 4°C, DAB staining.
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Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Mouse placenta probed with Rabbit Anti-GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | BIP; MIF2; GRP78; HEL-S-89n; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 7 kDa protein 5; Immunoglobulin heavy chain-binding protein; HSPA5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human GRP78 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC1, probably to facilitate the release of DNAJC1 from its substrate.
Additional Text
Gene Name
HSPA5
Gene ID
3309
Uniprot ID
P11021
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1219R Datasheet
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