GDF8/MSTN Polyclonal Antibody
Catalogue Number: BS-1288R-BSS
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Formalin-fixed and paraffin embedded mouse small intestine labeled Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Lane 1: Mouse Muscle lysates; Lane 2: Rat Muscle ysates; Lane 3: Rat Tongue lysates probed with GDF8/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDF8/MSTN ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDF8/MSTN ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Mouse skeletal muscle lysate probed with Anti GDF8/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 43kD. Observed band size: 43kD
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Generously provided by Bradley Elliott from University of Westminster as part of the Bioss Discovery Program. Lanes 1 and 2 are human muscle lysates and Lanes 3 and 4 are C2C12 cells. Rabbit Anti-GDF8/MSTN Polyclonal Antibody (bs-1288R) was used at a dilution of 1:1000, overnight at 4C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | GDF8; MSLHP; Growth/differentiation factor 8; GDF-8; Myostatin; MSTN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human GDF8 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. Acts specifically as a negative regulator of skeletal muscle growth. [SUBUNIT] Homodimer; [TISSUE SPECIFICITY] Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue. Belongs to the TGF-beta family.
Additional Text
Gene Name
MSTN
Uniprot ID
O14793
Gene ID
2660
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1288R Datasheet
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