FMRP (Ser500) Polyclonal Antibody
Catalogue Number: BS-13188R-BSS
BS-13188R Image
BS-13188R Image
BS-13188R Image
BS-13188R Image
BS-13188R Image
BS-13188R Image
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-13188R Image
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-13188R Image
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-13188R Image
Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-FMRP (Ser500) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
BS-13188R Image
Lane 1: mouse brain lysates probed with Rabbit Anti-FMRP (Ser500) Polyclonal Antibody, Unconjugated (bs-13188R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | POF; FMRP; POF1; FRAXA; Fragile X mental retardation protein 1; Protein FMR-1; FMR1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic phosphopeptide derived from human FMRP around the phosphorylation site of Ser500 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
Additional Text
Gene Name
FMR1
Uniprot ID
Q06787
Gene ID
2332
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Phosphorylated, Ser500
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-13188R Datasheet
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