Integrin Alpha V + Beta 5 Antibody
Catalogue Number: BS-1356R-BSS
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bs-1356R Image
bs-1356R Image
bs-1356R Image
bs-1356R Image
bs-1356R Image
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Integrin Alpha V + Beta 5 Polyclonal Antibody, Unconjugated (bs-1356R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Integrin Alpha V + Beta 5 Polyclonal Antibody, Unconjugated (bs-1356R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
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U2-OS cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin Alpha V + Beta 5 Antibody(bs-1356R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Formalin-fixed and paraffin embedded cervical cancer labeled with Anti-Integrin αV+β5 Polyclonal Antibody, Unconjugated (bs-1356R) followed by conjugation to the secondary antibody and DAB staining
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A549 cells probed with Rabbit Anti-Integrin Alpha V + Beta 5 Polyclonal Antibody (bs-1356R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CD51; MSK8; VNRA; VTNR; Integrin alpha-V; Vitronectin receptor subunit alpha; ITGAV |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Integrin beta 5 and Integrin alpha V |
| Application: | ELISA, FC, ICC, IF, IHC-P, IHC-Fr |
Description
Description: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Additional Text
Gene Name
ITGAV
Gene ID
3685
Uniprot ID
P06756
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1356R Datasheet
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