MAP2 Polyclonal Antibody
Catalogue Number: BS-1369R-BSS
BS-1369R Image
BS-1369R Image
BS-1369R Image
BS-1369R Image
BS-1369R Image
BS-1369R Image
BS-1369R Image
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
BS-1369R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
BS-1369R Image
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
BS-1369R Image
SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MAP2 Antibody(1369R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BS-1369R Image
Formalin-fixed and paraffin embedded rat brain labeled with Anti-MAP2/MAP-2A.B.C Polyclonal Antibody, Unconjugated (bs-1369R) followed by conjugation to the secondary antibody and DAPI staining
BS-1369R Image
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | MAP2A; MAP2B; MAP2C; Microtubule-associated protein 2; MAP-2; MAP2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human MAP2 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Additional Text
Gene Name
MAP2
Gene ID
4133
Uniprot ID
P11137
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
BS-1369R Datasheet
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