MAP2/MAP-2a.b.c Polyclonal Antibody
Catalogue Number: BS-1369R-BSS
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Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
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SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MAP2 Antibody(1369R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Formalin-fixed and paraffin embedded rat brain labeled with Anti-MAP2/MAP-2A.B.C Polyclonal Antibody, Unconjugated (bs-1369R) followed by conjugation to the secondary antibody and DAPI staining
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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | MAP2A; MAP2B; MAP2C; Microtubule-associated protein 2; MAP-2; MAP2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human MAP2 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Additional Text
Gene Name
MAP2
Gene ID
4133
Uniprot ID
P11137
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1369R Datasheet
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