CD177 Polyclonal Antibody
Catalogue Number: BS-1482R-BSS
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bs-1482R Image
bs-1482R Image
bs-1482R Image
Lane 1: Hela Cells; Lane 2: K562 Cells; Lane 3: MCF-7 Cells; Lane 4: 293T Cells; Lane 5: Sp2/0 Cells; Lane 6: NIH/3T3 Cells; 40ug of lysate loaded into each lane; Probed with CD177 Polyclonal Antibody, unconjugated (bs-1482R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti CD177/NB1 Polyclonal Antibody, Unconjugated (bs-1482R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
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Lane 1: Mouse Spleen lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat Lymph node lysates probed with CD177 Polyclonal Antibody, Unconjugated (bs-1482R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NB1 GP;PRV-1;HNA-2a;NB1 glycoprotein;Neutrophil Glycoprotein NB1; RGD1562941; CD177 antigen; Polycythemia rubra vera protein 1; PRV-1; NB1 glycoprotein; NB1 GP; Human neutrophil alloantigen 2a; HNA-2a; CD177; CD177_HUMAN; CD177_MOUSE. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat CD177 |
| Application: | IHC-P, WB |
Description
Description: Cd177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population. against NB1 can be involved in neonatal alloimmune neutropenia (NAN).
Additional Text
Gene Name
CD177
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Gene ID
499099
Modification
Unmodified
Uniprot ID
D3ZCH9
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1482R Datasheet
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