OCLN Polyclonal Antibody
Catalogue Number: BS-1495R-BSS
BS-1495R Image
BS-1495R Image
BS-1495R Image
BS-1495R Image
BS-1495R Image
BS-1495R Image
BS-1495R Image
BS-1495R Image
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
BS-1495R Image
Mouse Kidney lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
BS-1495R Image
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-1495R Image
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Antibody(bs-1495R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BS-1495R Image
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Polyclonal Antibody(bs-1495R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BS-1495R Image
Image provided by the Independent Validation Program, badge number 029632:Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-OCLN/Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:200 followed by conjugation to the secondary antibody and DAPI staining
BS-1495R Image
Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Ocl; AI53564; Occludin; Ocln |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse Occludin |
| Application: | FC, WB |
Description
Description: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Additional Text
Gene Name
OCLN
UniProt ID
Q61146
Purification
Protein A purified
Gene ID
18260
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
BS-1495R Datasheet
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