EpCAM/CD326 Polyclonal Antibody
Catalogue Number: BS-1513R-BSS
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Formalin-fixed and paraffin-embedded human rectal carcinoma labeled with Rabbit Anti-EpCAM Polyclonal Antibody (bs-1513R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
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Mouse kidney Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining with EpCAM Polyclonal Antibody, Unconjugated (bs-1513R) was performed at 1:100 for 30 min on ice. The secondary antibody, Goat anti-rabbit IgG-PE, was used at a 1:200 dilution for 30 minutes on ice. Primary antibody staining (green) is compared to compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).
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Lane 1: A431 cell lysates; Lane 2: MCF-7 cell lysates probed with SIRT4 Polyclonal Antibody, Unconjugated (bs-1513R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: HepG2 lysates probed with EpCAM/CD326 Polyclonal Antibody, Unconjugated (bs-1513R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with EpCAM/CD326 Polyclonal Antibody, Unconjugated (bs-1513R) at 1:400 overnight at 4°C, DAB staining.
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Lane 1: Mouse Small intestine lysates; Lane 2: Mouse Large intestine lysates probed with EpCAM/CD326 Polyclonal Antibody, Unconjugated (bs-1513R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Human A431 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human K562 cell lysates probed with EpCAM/CD326 Polyclonal Antibody, Unconjugated (bs-1513R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with EpCAM/CD326 Polyclonal Antibody(bs-1513R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with EpCAM/CD326 Polyclonal Antibody(bs-1513R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Paraformaldehyde-fixed, paraffin embedded (mouse prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (bs-1513R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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L1 rat heart, L2 rat brain lysates probed (bs-1513R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted and observed band size: 35kDa.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human EpCAM |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Additional Text
Gene ID
4072
Gene Name
EPCAM
Uniprot ID
P16422
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP4; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; hEGP314; KS 1/4 antigen; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; GA733-2; M1S2
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1513R Datasheet
SUPPORT
outstanding technical support
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DELIVERY
we offer free delivery to UK universities and non profit organisations