IL-6R alpha Polyclonal Antibody
Catalogue Number: BS-1805R-BSS
bs-1805R Image
bs-1805R Image
bs-1805R Image
bs-1805R Image
bs-1805R Image
bs-1805R Image
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-6R alpha Polyclonal Antibody, Unconjugated (bs-1805R) at 1:400 overnight at 4°C, DAB staining.
bs-1805R Image
U-937 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-6R alpha Antibody (bs-1805R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
bs-1805R Image
Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti IL-6R alpha/IL-6 receptor α Polyclonal Antibody, Unconjugated (bs-1805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
bs-1805R Image
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-6R alpha Polyclonal Antibody(bs-1805R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
bs-1805R Image
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-6R alpha Polyclonal Antibody(bs-1805R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Il6r; CD126; IL-6R; Interleukin-6 receptor subunit alpha; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; Il6ra |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse IL-6R alpha |
| Application: | ELISA, FC, ICC, IF, IHC-P, IHC-Fr |
Description
Description: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Additional Text
Gene ID
16194
Purification
Protein A purified
Uniprot ID
P22272
Gene Name
Il6ra
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1805R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations