PDL2/B7-DC Polyclonal Antibody
Catalogue Number: BS-1868R-BSS
BS-1868R Image
BS-1868R Image
BS-1868R Image
BS-1868R Image
Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-B7-DC/CD273/PDL2, Unconjugated (bs-1868R) followed by conjugation to the secondary antibody
BS-1868R Image
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PDCD1LG2) polyclonal Antibody, Unconjugated (bs-1868R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
BS-1868R Image
HepG2 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDCD1LG2 Polyclonal Antibody(bs-1868R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; Programmed cell death 1 ligand 2; PD-1 ligand 2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; PDCD1LG2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human B7-DC |
| Application: | ELISA, IHC-P |
Description
Description: Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).
Additional Text
Uniprot ID
Q9BQ51
Gene ID
80380
Gene Name
PDCD1LG2
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1868R Datasheet
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