S100B Polyclonal Antibody
Catalogue Number: BS-2015R-BSS
BS-2015R Image
BS-2015R Image
BS-2015R Image
BS-2015R Image
BS-2015R Image
BS-2015R Image
BS-2015R Image
BS-2015R Image
Formalin-fixed and paraffin embedded rat brain labeled with Anti-S100B Polyclonal Antibody, Unconjugated (bs-2015R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
BS-2015R Image
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-S100B Polyclonal Antibody, Unconjugated(bs-2015R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
BS-2015R Image
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100B) Polyclonal Antibody, Unconjugated (bs-2015R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-2015R Image
Paraformaldehyde-fixed, paraffin embedded (Human glioblastoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100B) Polyclonal Antibody, Unconjugated (bs-2015R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-2015R Image
Paraformaldehyde-fixed, paraffin embedded (Human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100B) Polyclonal Antibody, Unconjugated (bs-2015R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-2015R Image
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100B) Polyclonal Antibody, Unconjugated (bs-2015R ) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-2015R Image
Lane 1,2: Recombinant human S100B protein, N-His probed with S100B Polyclonal Antibody, Unconjugated (bs-2015R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NEF; S1; S1-B; S1beta; Protein S1-B; S-1 protein beta chain; S-1 protein subunit beta; S1 calcium-binding protein B; S1B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human S100B |
| Application: | ELISA, ICC, IF, IHC-P, IHC-Fr |
Description
Description: Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Additional Text
Gene Name
S100B
Uniprot ID
P04271
Gene ID
6285
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
BS-2015R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations