Klotho Polyclonal Antibody
Catalogue Number: BS-2925R-BSS
bs-2925R Image
bs-2925R Image
bs-2925R Image
bs-2925R Image
bs-2925R Image
bs-2925R Image
bs-2925R Image
BS-2925R Image
bs-2925R Image
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
bs-2925R Image
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
bs-2925R Image
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.
bs-2925R Image
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.
bs-2925R Image
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.
bs-2925R Image
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.
bs-2925R Image
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
BS-2925R Image
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HL-60 cell lysates probed with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Klotho; KL |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Klotho |
| Application: | IF, IHC-P, IHC-Fr |
Description
Description: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
Additional Text
Gene ID
9365
Uniprot ID
Q9UEF7
Gene Name
KL
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-2925R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations