phospho-Bax (Ser184) Polyclonal Antibody
Catalogue Number: BS-3010R-BSS
BS-3010R Image
BS-3010R Image
BS-3010R Image
BS-3010R Image
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4°C, DAB staining.
BS-3010R Image
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei
BS-3010R Image
Lane 1:mouse brain lysates probed with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184 |
| Application: | ELISA, FC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Additional Text
Gene Name
BAX
Gene ID
581
Uniprot ID
Q07812
Purification
Protein A purified
Modification
Phosphorylated, Ser184
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
BS-3010R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations