FAK (Tyr397) Polyclonal Antibody
Catalogue Number: BS-3159R-BSS
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bs-3159R Image
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bs-3159R Image
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Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Rat Adrenal gland lysates; Lane 6: Rat Testis lysates; Lane 7: Human 293T cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human HUVEC cell lysates; Lane 10: Human MDA-MB-231 cell lysates; Lane 11: Human A549 cell lysates probed with FAK (Tyr397) Polyclonal Antibody, Unconjugated (bs-3159R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAK (Tyr397) Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 overnight at 4°C, DAB staining.
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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-GnRHR Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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L1 mouse intestine lysate L2 rat lung lysates probed with Anti Phospho-FAK (Tyr397)Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 119kD. Observed band size: 55kD, 60kD, and 119kD
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH cunjugated synthetic phosphopeptide derived from human FAK 1 around the phosphorylation site of Tyr397 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]
Additional Text
Gene Name
PTK2
Gene ID
5747
Uniprot ID
Q05397
Modification
Phosphorylated, Tyr397
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-3159R Datasheet
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