IRF3 (Ser396) Polyclonal Antibody

Catalogue Number: BS-3195R-BSS

Manufacturer:Bioss Inc
Shelf Life:12 months
Type:Polyclonal Primary Antibody - Unconjugated
Alias:Interferon regulatory factor 3; IRF-3; IRF3
Shipping Condition:Blue Ice
Unit(s): 100 ul, 20 ul
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396
Application: ELISA, ICC, IF, IHC-P, WB, IHC-Fr

Description

Description: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Additional Text

Gene Name

IRF3

Gene ID

3661

Uniprot ID

Q14653

Modification

Phosphorylated, Ser396

Purification

Protein A purified

Concentration

1 ug/ul

Antibody Clonality

Polyclonal

Storage Note

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.