tubulin Beta Polyclonal Antibody

Catalogue Number: BS-4511R-BSS

BS-4511R Image

Formalin-fixed and paraffin embedded rat brain labeled with Anti-tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

BS-4511R Image

Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.

BS-4511R Image

Mouse ovary lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BS-4511R Image

Lane 1: Cerebrum lysates probed with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BS-4511R Image

Lane 1: Uterus lysates probed with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BS-4511R Image

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, DAB staining.

BS-4511R Image

Lane 1: SiHa cell lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Heart lysates ; Lane 5: Mouse Heart lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BS-4511R Image

Lane 1: Hela cell lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: NIH/3T3 cell lysates; Lane 4: Mouse Cerebrum lysates ; Lane 5: HL60 cell lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Cerebellum lysates; Lane 8: Mouse Heart lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BS-4511R Image

HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Beta tubulin Polyclonal Antibody(bs-4511R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

BS-4511R Image

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, DAB staining.

BS-4511R Image

Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, DAB staining.

BS-4511R Image

Tissue/cell:Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beta tubulin) polyclonal Antibody, Unconjugated (bs-4511R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

BS-4511R Image

Lane 1: Mouse Cerebellum lysates; Lane 2: Rat Cerebellum lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Mouse Heart lysates; Lane 7: Rat Heart lysates probed with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BS-4511R Image

Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Beta tubulin (bs-4511R) at 1/1000~20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

BS-4511R Image

Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Beta tubulin (bs-4511R) at 1/1000~20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

BS-4511R Image

Sample: MDA-MB-231 (Human) Lysate at 40 ug Primary: Anti-Beta tubulin (bs-4511R) at 1/2000~20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

BS-4511R Image

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (tubulin Beta) Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BS-4511R Image

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (tubulin Beta) Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BS-4511R Image

Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (tubulin Beta) Polyclonal Antibody, Unconjugated (bs-4511R-3) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BS-4511R Image

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (tubulin Beta) Polyclonal Antibody, Unconjugated (bs-4511R-3) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BS-4511R Image

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37°C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37°C

BS-4511R Image

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 50kD. Observed band size: 50kD.

Previous Next

Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	M4; TUBB1; TUBB5; CDCBM6; OK/SW-cl.56; Tubulin beta chain; Tubulin beta-5 chain; TUBB
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	KLH conjugated synthetic peptide derived from human tubulin Beta
Application:	ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.