MBD4 Polyclonal Antibody
Catalogue Number: BS-53050R-BSS
BS-53050R Image
BS-53050R Image
BS-53050R Image
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human MBD4 (bs-53050R) in antigen-coated wells. The wells were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:15,000.
BS-53050R Image
(Left) U2OS cells were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MBD4 (bs-53050R) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. (Right) Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 µg) with the antibody against MBD4 (bs-53050R), diluted 1:2,000 in TBST containing 3% milk powder.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Preservative: | 0.05% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | MED1, Methyl-CpG-binding domain protein 4, MBD4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides |
| Application: | ELISA, WB |
Description
Description: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Additional Text
Gene Name
MBD4
Uniprot ID
O95243
Gene ID
8930
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bs-53050R Datasheet
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