EHMT1 Polyclonal Antibody
Catalogue Number: BS-53068R-BSS
BS-53068R Image
BS-53068R Image
BS-53068R Image
To determine the titer, an ELISA was performed using a serial dilution of the EHMT1 antibody (bs-53068R). The wells were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.
BS-53068R Image
Nuclear extracts of HeLa cells (40 µg) were analyzed by Western blot using the EHMT1 antibody (bs-53068R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment may represent a splicing variant.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Preservative: | 0.05% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Histone-lysine N-methyltransferase EHMT1, EHMT1, GLP1, Lysine N-methyltransferase 1D, H3-K9-HMTase 5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Polyclonal antibody raised in rabbit against mouse Ehmt1, using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein |
| Application: | ELISA, IHC-P, WB |
Description
Description: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
Additional Text
Gene Name
EHMT1
Uniprot ID
Q9H9B1
Gene ID
79813
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bs-53068R Datasheet
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