DGCR8 Polyclonal Antibody
Catalogue Number: BS-53088R-BSS
BS-53088R Image
BS-53088R Image
To determine the titer of the antibody, an ELISA was performed using a serial dilution of DGCR8 antibody (bs-53088R). The plates were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,100.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Preservative: | 0.05% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | DGCRK6, C22orf12, Microprocessor complex subunit DGCR8, DGCR8, LP4941 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Polyclonal antibody raised in rabbit against mouse DGCR8 using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively |
| Application: | ELISA |
Description
Description: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal.
Additional Text
Gene Name
DGCR8
Uniprot ID
Q8WYQ5
Gene ID
54487
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bs-53088R Datasheet
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