HDAC1 Polyclonal Antibody
Catalogue Number: BS-55093R-BSS
BS-55093R Image
BS-55093R Image
BS-55093R Image
BS-55093R Image
BS-55093R Image
BS-55093R Image
Lane 1: 293T cell lysates; Lane 2: HDAC1 knockout (KO) 293T cell lysates probed with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-55093R Image
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HDAC1) polyclonal Antibody, Unconjugated (bs-55093R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
BS-55093R Image
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:100 overnight at 4°C, DAB staining.
BS-55093R Image
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:100 overnight at 4°C, DAB staining.
BS-55093R Image
Lane 1: Human 293T cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Mouse Thymus lysates; Lane 6: Mouse Testis lysates; Lane 7: Rat Thymus lysates; Lane 8: Rat Testis lysates probed with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | GON-10;HD1;RPD3;RPD3L1;HDAC1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2). |
| Application: | ELISA, FC, IF, IHC-P, IP, WB, IHC-Fr |
Description
Description: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Additional Text
Gene Name
HDAC1
Gene ID
3065
Uniprot ID
Q13547
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bs-55093R Datasheet
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