HMGB1 Polyclonal Antibody
Catalogue Number: BS-55098R-BSS
BS-55098R Image
BS-55098R Image
BS-55098R Image
BS-55098R Image
BS-55098R Image
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, DAB staining.
BS-55098R Image
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, DAB staining.
BS-55098R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, DAB staining.
BS-55098R Image
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Human Jurkat cell lysates ; Lane 6: Human Hela cell lysates ; Lane 7: Human HepG2 cell lysates ; Lane 8: Human Molt-4 cell lysates probed with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human HMGB1 |
| Application: | IF, IHC-P, WB |
Description
Description: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Additional Text
Gene ID
3146
Gene Name
HMGB1
Uniprot ID
P09429
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; High mobility group protein B1; HMG3; HMGB 1;HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
BS-55098R Datasheet
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DELIVERY
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